Bordetella pertussis - PCR
Test Identifier Information
Bordetella pertussis DNA detection. Nucleic acid extraction, PCR amplification and detection.
|External Price||$92.37(Exclusive of GST)|
|Specimen Collection Protocols|
Preferred specimen is a dry swab, however a swab in VTM is also an acceptable specimen.
|Patient Specimen||Dry nasopharyngeal/pernasal swab. Nasopharyngeal aspirate|
|Paediatric Specimen||As above|
|Sample Delivery to Lab||Dry swab or NPA in original container at room temperature|
|Aliquot Transport to CHL||Place specimen in specimen bag.|
|Contact Phone Number||(03) 364 0356 | x80356|
|Test Availability||Batched Mon - Fri, 0900 hrs - urgent testing by arrangement|
|Turnaround Time||7 - 75 hours|
PCR offers a highly sensitive and specific technique for diagnostic testing and allows for same day results.PCR is more sensitive than and remains positive longer than culture. PCR is more likely than culture to detect infection in individuals treated with antibiotics.
1. Clinical pertussis infection in an unimmunised individual has an incubation period of 7 - 10 days (range 6 - 20 days).
2. The first symptomatic or catarrhal stage exhibits non-specific symptoms consistent with many different upper respiratory tract infections (URTI) and it is difficult to clinically distinguish infection from other causes of URTIs. During this stage the organisms is highly communicable and most readily isolated from the posterior nasopharynx. Antibiotics can be effective at modifying the course of disease at this stage.
3. The paroxysmal phase follows the catarrhal stage and exhibits a dry non-productive cough culminating in a whoop with or without vomiting. This may persist for up to 4 weeks. There is a decrease in the number of bacteria, and antibiotics will have little or no effect at modifying the disease at this stage.
4. Complications can include otitis media, pneumonia, toxic nervous system manifestations and pneumothorax.
5. Bordetella pertussis is a fastidious organism requiring specialised growth conditions, and bacterial isolation is also reduced with an advancing disease state. DNA detection by PCR detects Bordetella pertussis with increased sensitivity and over a longer period of the disease compared to culture.
6. The PCR is able to detect one CFU (Colony Forming Unit) / millilitre. Sensitivity is increased if sampling is performed before 10 days of symptoms has elapsed
|Delphic Number Test Number||2532|