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Test Identifier Information

Registration CodeCJDP

Immunoassay of 14-3-3 protein in the CSF.

External Price$1350.13(Exclusive of GST)

Specimen Collection

Pre-Testing Requirements

Lab must be phoned & clinical information MUST be provided. Refer written protocols

If PCR testing for viruses and bacteria is requested and CJD is also requested the 14-3-3 protein test will be performed first. This may delay the PCR testing for 1-2 weeks. 

Note: Requestors are advised that referred specimens for 14-3-3 protein are sent on week 1 and week 3 of the month. TAT's advised below.

Specimen Collection Protocols

1) Minimum 2mL of CSF - undiluted (CSF must be clear and colourless)

2) Please provide routine culture counts, protein levels and clinical information (see
additional information). These are needed for interpretation.
3) Unsuitable samples will be rejected.
CAUTION Specimen handling (please label request form to alert lab staff)
4) Precautions should be taken when handling specimens suspected of containing
agents that cause CJD. Prions are relatively resistant to routine forms of
laboratory disinfection. Gloves should always be worn and disposable equipment
should be used where possible.
Unsuitable samples:
1) Macroscopically haemorrhagic samples
2) Xanthochromic samples
3) Red blood cell counts >500 cells per μL
4) White cells counts >10 cells per μL
5) 14-3-3 protein exists in erythrocytes, platelets and plasma. Lysis of these cells
releases the protein thus contaminating the CSF sample and leading to false
positive results.
Patient SpecimenSee specimen collection protocol below
Sample Delivery to LabAt 4C (on wet ice or chilly pad)

CHLabs Laboratory

Contact Details** Unknown email address **
Contact Phone Number03-3640416 / ext. 80416
Test AvailabilitySent to Melbourne University, CJD registry
Turnaround Time1 - 2 weeks
Referred toFlorey Institute - week 1 and week 3 of the month
Reference Interval

For application refer to Interpretation.


The 14-3-3 protein test is a non-specific marker of CNS system injury or death. In carefully selected patients, a positive result can have approximately 90% sensitivity and specificity for sporadic Creutzfeldt-Jakob disease.
Testing should only be performed in the appropriate clinical setting of the patient with dementia who has not had a cerebral infarct in the last month.
False positive results have been noted in encephalitis (especially Herpes simplex virus) and recent cerebral infarcts.

Additional Information

Routine cell counts and protein levels must be provided. These results are needed for interpretation.

IMPORTANT: clinical information must be provided. Please indicate onset, history of dementia, any tests suggesting CJD i.e. MRI or EEG. Mention of other indicators - rigitity, myoclonus, ataxia. If the patient has none of these, an outline of what is indicated is needed.

A copy of the original doctors request slip is required to accompany the sample.

Delphic Number Test Number2327

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