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A B C D E F G H I J K L M N O P Q

Test Identifier Information

 
Registration CodeMONC
Method

The AmpFlSTR PCR Amplification Kit is a short tandem repeat (STR) multiplex assay that amplifies 15 tetranucleotide repeat loci (D8S1179, D21S11, D7S820, CSF1PO, D3S1358, THO1, D13S317, D16S539, D2S1338, D19S433, vWA, TPOX, D18S51, D5S818, FGA) and the Amelogenin gender determining marker in a single PCR amplification.  

Diagnostic Use / Indications

Short tandem repeats (STR) of 2-4 bp in genomic DNA are often highly variable with regard to the number of repeats present and can be used to distinguish the DNA of one person from that of another. They are useful for monitoring the success of bone marrow transplants by showing whether the cells present in blood or bone marrow are those of the donor or recipient. Primers specific to sequences flanking the repeats are used to amplify across the variable repeat regions, giving products which differ in size. Primer pairs are used to screen for informative alleles i.e. differ in size between the donor and recipient.

External Price$792.77(Exclusive of GST)
  

Specimen Collection

 
Pre-Testing Requirements

 

 

 

Prior arrangements with the laboratory are required prior to the sending of samples.  Please coordinate with the Genetics team on 03 364 0300 ext 86008.

Advanced notice of sample arrival and collection early in the day is required to accommodate the lengthy processing time to stabilise the samples.

Note: Donor and pre-transplant recipient (patient) DNA samples should be tested for informative alleles prior to chimaerism analysis.  These prior tests incurr an additional costs for DNA extraction and informative allele screens by STR analysis.  If no pre-transplant sample is available, it is possible to determine pre-transplant informative alleles from DNA isolated from a buccal scraping sample.

Patient SpecimenBlood or Bone Marrow.
Sample Delivery to LabAmbient temperature within 24 hours of collection.
  

Instructions for Referral to CHLabs

 
Aliquot Instructions30 mL peripheral blood in EDTA. 1-3 mL bone marrow in EDTA or RPMI (with anticoagulant).
Aliquot Transport to CHLUrgent courier at room temperature, within 24 hours of collection.
  

CHLabs Laboratory

 
DepartmentBiochemistry - Molecular Oncology
Contact Details Email Email
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Contact Phone Number03 3786 008 / x86008
Turnaround Time3-5 days
Additional Information

Delphic Number Test Number7903

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