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A B C D E F G H I J K L M N O P Q

Test Identifier Information

 
Registration CodeCTGN
Method

Fluorescence in situ hybridisation (FISH) on formalin fixed paraffin embedded (FFPE) tissue slides using a breakapart probe specific for the ALK rearrangement.

Diagnostic Use / Indications

ALK is the abbreviated term for anaplastic large-cell lymphoma kinase, which is part of the Insulin receptor superfamily . The precise function of the gene is not well understood. However, ALK rearrangements occur in 2-7% of patients and these people respond to treatment with crizotinib, which has been FDA approved. The most common aberrant fusion gene resulting from an ALK rearrangement is the EML4-ALK fusion gene, but the ALK break-apart probe will detect all rearrangements of ALK.

External Price$518.68(Exclusive of GST)
  

Specimen Collection

 
Pre-Testing Requirements

Please call the laboratory in advance if there is particular urgency for a result.

 

Specimen Collection Protocols
  •  

    Formalin Fixed Paraffin Embedded Tissue section (2–4 microns thick) should be placed on positively -charged, poly-l-lysine coated slides.
    Samples de-calcified in Formic acid are not suitable for FISH as this process affects the integrity of the DNA.
  • Ensure one H&E slide and 2-3 unprocessed slides are supplied for FFPE Tissue FISH. All slides must be clearly labelled with two patient identifiers identical to the request form.
  • The H&E slide must be assessed by a pathologist prior to receipt, and appropriate areas for FISH analysis should be clearly marked.
Patient SpecimenParaffin embedded tumour section slides
Sample Delivery to LabIn slide-holders or boxes at room temperature.
  

CHLabs Laboratory

 
DepartmentHaematology - Cytogenetics
Contact Details Email Email
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Contact Phone Number03 3786 007 ext 86007
Test AvailabilitySamples are processed 3 times per week, during business hours
Turnaround TimeUrgent 1-3 days. Routine 5 days
Interpretation

ALK rearrangement is reported if more than 15% of cells examined show a signal pattern consistent with a clinically significant ALK rearrangement.

Specimens are analysed and reported in accordance with CAP/ASCO recommendations (2013). 

Rearrangements and copy number changes   which have no proven clinical impact may also be detected using this probe. These will not be reported.

Delphic Number Test Number6085

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