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A B C D E F G H I J K L M N O P Q

Test Identifier Information

 
Registration CodeCTGN
Method

Fluorescence In Situ Hybridisation (FISH) is a molecular cytogenetic technique that can be described as a’ hybrid’ of molecular genetic and conventional cytogenetic technologies. The technique combines the ability to identify a specific gene or gene region with direct visualisation of cells and/or chromosomes under the microscope.

FISH is usually applied to standard cytogenetic preparations, on glass slides and both cultured and uncultured cells can be used, unlike standard cytogenetic techniques which require actively-dividing cells. This means that a variety of specimen types can be processed successfully, even those that conventionally don’t grow well in culture, making FISH a powerful tool for the study of acquired abnormality in bone marrows, tumours and even paraffin-embedded specimens. As a complementary technique, or as a test for targeted abnormalities, FISH has expanded our capabilities for more accurate, rapid and refined cytogenetic diagnoses.

Diagnostic Use / Indications

 

Available tests include (but not limited to):

 

 

TP53 deletion

IGH-FGFR3 Fusion

MYC Rearrangement

13q14.3 deletion

IGH-CCND1 Fusion

CBFB Rearrangement

MYCN Enumeration

IGH-MAF Fusion

ETV6/RUNX1 Fusion

D20S108 (20q) deletion

IGH-BCL2 Fusion

ATM Deletion

Various chromosome enumeration

PML-RARA Fusion

KMT2A(MLL) Rearrangement

 

Current FISH Panels include:

 

Panel

Tests

Paediatric ALL Panel

ETV6-RUNX1 fusion

4 & 10 enumeration (Double Trisomy)

BCR-ABL1 fusion

MLL rearrangement

CLL Panel

ATM deletion

Trisomy 12

13q14.3 deletion

TP53 deletion

MM Panel

IGH-FGFR3 fusion

TP53 deletion

 

 

External Price$279.13(Exclusive of GST)
  

Specimen Collection

 
Specimen Collection Protocols

Send all specimens at room temperature

Transport medium is available from the laboratory by contacting LabInfo@cdhb.health.nz

 

Tissue

 

Bone Marrow

    • >1ml bone marrow aspirate in transport medium
    • Bone marrow aspirate smear slide (2x slides)

Peripheral Blood

    • At diagnosis: 5-10mL Lithium Heparin (green top)
    • Minimal residual disease: 10mL Lithium Heparin (green top)

    Note: Whole blood is required for genetic testing; please DO NOT centrifuge blood tubes

Tumour/Lymph Node

>1cm tumour/lymph node tissue in transport medium

Tumour touch preparation slide (2x slides)

Patient Specimen1-2ml blood in lithium heparin/bone marrow in RPMI
Sample Delivery to Laboom temperature. DO NOT refrigerate, freeze or centrifuge
  

CHLabs Laboratory

 
DepartmentHaematology - Cytogenetics
Contact Details Email Email
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Contact Phone Number03 3786 007 ext 86007
Test AvailabilityMon-Fri, 8am-5pm. Urgent testing outside these hours by arrangement
Turnaround TimeUrgent 1-3 days; Routine 2 -18 days
Reference Interval

Normal and abnormal ranges have been established in-house as part of the validation process for each probe. Please contact the laboratory for further details.

Uncertainty of Measurement

Please contact the laboratory if you have specific queries regarding our testing.

Additional Information

An additional charge for direct interphase preparation applies where cell culture, harvest and hold or chromosome analysis has not also been requested.


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