skip navigation
A B C D E F G H I J K L M N O P Q

Test Identifier Information

 
Registration CodeMONC
Method

Allele-specific PCR to detect the two most common somatic MPL mutations associated with myeloproliferative neoplasms (Takei, H. et al., PLoS ONE 9(8): e104958).

NM_005373.2(MPL):c.1544G>T p.(Trp515Leu) commonly referred to as MPL W515L

NM_005373.2(MPL):c.1543_1544delinsAA p.(Trp515Lys) commonly referred to as MPL W515K
 

 


 

Diagnostic Use / Indications

MPL encodes the thrombopoietin receptor which is important for the proliferation of megakaryocytes that produce platelets.  Some MPL mutations result in a constitutively activated receptor which leads to an increase in platelet number (essential thrombocythaemia) and/or stimulation of other bone marrow cells to release collagen (primary myelofibrosis).  These MPL mutations are included in the WHO 2016 major criteria for diagnosis of PMF and ET.

External Price$253.75(Exclusive of GST)
  

Specimen Collection

 
Pre-Testing Requirements

JAK2 V617F and CALR mutation analysis should be performed before ordering MPL mutation testing on patients with clinical indicators of essential thrombocythemia or primary myelofibrosis.  We store all extracted DNA - please contact the laboratory if you would like MPL mutation testing performed on the same DNA used for JAK2 V617F and CALR mutation testing.

Patient Specimen4 ml peripheral blood (EDTA)
Sample Delivery to LabSend to the laboratory at ambient temperature or 4 C.
  

CHLabs Laboratory

 
DepartmentBiochemistry - Molecular Oncology
Contact Details Email Email
This e-mail address is being protected from spam bots, you need JavaScript enabled to view it
Contact Phone Number03 3786 008 / x86008
Turnaround Time28 days
Reference Interval

 Not applicable.

Interpretation

 Detection of a MPL mutation is consistent with diagnosis of a myeloproliferative neoplasm.

Uncertainty of Measurement

Limit of Detection: 1% mutant MPL copies over total MPL gene copies.



 


CHL Follow us on Facebook