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A B C D E F G H I J K L M N O P Q

Test Identifier Information

 
Registration CodeANCA
Method

Indirect Immunofluorescence on formalin fixed neutrophils.

ANCA are screened at 1 in 20.

External Price$34.50(Exclusive of GST)
  

Specimen Collection

 
Pre-Testing Requirements

None

Patient Specimen5 mL clotted blood (Red)
Paediatric Specimen0.5 mL clotted blood (Red)
Sample Delivery to LabRoom temperature
  

Instructions for Referral to CHLabs

 
Aliquot Instructions100 uL serum - minimum 1 mL serum - preferred Store in fridge
Aliquot Transport to CHLRoom temperature
  

CHLabs Laboratory

 
DepartmentBiochemistry - Immunology
Contact Details Email Email
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Contact Phone Number(03) 364 0414 | x80414
Test AvailabilityMon - Fri, business hours only
Turnaround Time3 days
Reference Interval

- Not detected
- Positive and pattern

Interpretation

Anti-neutrophil Cytoplasmic Antibody

Cytoplasmic ANCA (c-ANCA) is typically present in 80-100% of patients with generalised active Wegener’s Granulomatosis (WG), and in a lesser percentage in vasculitic disease within the clinical spectrum of Wegener's Granulomatosis. Antibodies producing the cytoplasmic pattern (c-ANCA) most often react with the azurophilic granule enzyme, proteinase 3 (PR3). However up to 15% of cANCA positive sera have other antigen specificities that at the time of writing have no recognised clinical utility. Antibodies producing the perinuclear pattern (p-ANCA) vary to an even greater extent in their antigen specificity (anti-myeloperoxidase (MPO), anti-elastase, anti-lactoferrin amongst others). As currently only PR3-ANCA and MPO-ANCA have clinical utility it is necessary to run specific assays to define the antibody specificity of all ANCA positive sera. Any serum producing a c-ANCA, p-ANCA or atypical ANCA pattern will be assayed for MPO-ANCA and PR3-ANCA using an ELISA. MPO-ANCA and PR3-ANCA are specific for vasculitic diseases within the clinical spectrum of WG and microscopic polyangiitis although the spectrum of disease associated with these antibodies is expanding.

Delphic Number Test Number6845

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