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A B C D E F G H I J K L M N O P Q

Test Identifier Information

 
Registration CodeMONC
Method

IGH PCR amplification is carried out using primers homologous to sequences of all three conserved framework regions (FR1, FR2 and FR3), and the joining (J) region of the immunoglobulin heavy chain (IGH) gene.

 

Sensitivity approximately 2%.

Diagnostic Use / Indications

For the detection or exlcusion of clonal B cell populations.

Constituent TestsDNA extraction and storage ;
External Price$259.21(Exclusive of GST)
  

Specimen Collection

 
Specimen Collection Protocols

Aseptic Technique.

Patient SpecimenBlood, Bone marrow or Tissue.
Sample Delivery to LabFrozen tissue to be kept frozen, otherwise room temperature.
  

Instructions for Referral to CHLabs

 
Aliquot Instructions10 ml peripheral blood in EDTA. 1-3 mL of bone marrow in EDTA or RPMI (anticoagulant). Fresh or frozen tissue (5mm cube). Paraffin-embedded tissue: block or at least 10 x 10 micron slices.
Aliquot Transport to CHLCourier
  

CHLabs Laboratory

 
DepartmentBiochemistry - Molecular Oncology
Contact Details Email Email
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Contact Phone Number03 3786 008 / x86008
Test AvailabilityMon - Fri, business hours - urgent testing by arrangement.
Turnaround Time7 days
Interpretation

Clonal populations of cells yield one (monoclonal), two (clonal), or more (oligoclonal)  prominent amplicons within a specified size range.  A normal (polyclonal) population of B cells will produce a polyclonal smear representing a heterogeneous population of V-J region rearrangements and, if graphed, would resemble a bell-shaped Gaussian curve.

Additional Information

Paraffin-embedded tissue samples may, or may not, have DNA of a suitable quality or quantity for this assay and may be reported as Insufficient.

Delphic Number Test Number6922

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