skip navigation
A B C D E F G H I J K L M N O P Q

Test Identifier Information

 
Registration CodeSPEP
Method

Electrophoresis: - Separation of proteins in an electric field based the protein charge density.

Immunofixation: - Anti-sera specific precipitation of electrophoretically separated proteins, permitting identification.

Nephelometric Quantitation: - The measurement of immunoglobulin heavy chains identified by immunofixation, using anti-body specific turbidimetric measurement.

Diagnostic Use / Indications

Immunofixation is used to determine the identity of abnormal bands visible on electrophoresis. Abnormal bands may include monoclonal bands, protein isoforms or abnormal proteins.

Used with other tests to assist with the identification of multiple myeloma.

External Price$58.91(Exclusive of GST)
  

Specimen Collection

 
Specimen Collection Protocols

2 mL of blood should be collected into a plain tube (red top) and serum separated off for analysis.

Plasma samples may interfere with immunofixation due to cross-reaction with fibrinogen, obscuring interpretation.

Patient SpecimenBlood 2 mL Plain (T15) (Red)
  

Instructions for Referral to CHLabs

 
Aliquot Instructions0.5 mL Serum
Aliquot Transport to CHLASAP - on ice or ambient
  

CHLabs Laboratory

 
DepartmentBiochemistry - Protein Laboratory
Contact Details Email Email
This e-mail address is being protected from spam bots, you need JavaScript enabled to view it
Contact Phone Number(03)3640334 | x80334
Test AvailabilityMonday - Friday, 8.00 am - 3.30 pm
Turnaround Time1 - 3 days
Reference Interval

Refer to Protein Chemistry Lab ext 80334

Interpretation

No monoclonal band detected - Immunofixation did not identify any protein. There is no band visible on electrophoresis. In some instances patients with monoclonal free light chains may have no detectable monoclonal band. This is rare but has been observed.

Small band not clearly identified - A small band is evident on electrophoresis, but is not identified with immunofixation.

Small band of possible ... - A small band is identified on electrophoresis and is vaguely detected on immunofixation. Identity is uncertain.

Monoclonal band detected, identified as ... - A distinct band is identified on electrophoresis and is clearly identified on immunofixation and reported. More than one band may be identified.

Bis-transferrin noted - A variant of transferrin is observed on electrophoresis and is confirmed on immunofixation. A record is kept for future laboratory reference. (Transferrin here is used as a common example)

Plasma sample only received ... - Plasma only had been taken. Results that are presented are unable to account for what may be masked by the presence of fibrinogen.

Delphic Number Test Number3212

CHL Follow us on Facebook