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A B C D E F G H I J K L M N O P Q

Test Identifier Information

 
Registration CodeTPP
Method

HPLC following production of fluorescent product

External Price$61.41(Exclusive of GST)
  

Specimen Collection

 
Pre-Testing Requirements

Nil

Specimen Collection Protocols

A 5 ml non gel heparin tube is the prefered sample type.

If other biochemistry is requested requiring a heparin specimen, please collect a separate tube for the vitamin B1  

Patient SpecimenBlood 5 mL heparin (T34). non gel tube prefered
Sample Delivery to LabAmbient
  

Instructions for Referral to CHLabs

 
Aliquot Instructions2 mL whole blood, to be sent to Specialist lab urgently, via hoist (phone 80332). After hours prepare in Separating area as per protocol. Wash with NaCL, dil cells1/1with dist H2O and freeze.
Aliquot Transport to CHLSend frozen prepared haemolysed cells
  

CHLabs Laboratory

 
DepartmentBiochemistry - Specialist
Contact Details Email Email
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Contact Phone Number03 364 0332
Test AvailabilityBatched fortnightly usually Tuesday
Turnaround Time1 day - 3 weeks
Reference Interval

Recommended Range >140 nmol/L

Additional Information

The red blood cells must be washed, haemolysed and frozen before they are 4 hours old (post venipuncture). Samples for analysis of thiamine are prepared by washing the red cells with isotonic 0.9% saline, preparing a red cell lysate and freezing.

METHOD

  1. Centrifuge the sample at 3000 rpm for 5 minutes.
  2. After centrifugation use a pasteur pipette to remove and discard the plasma and buffy coat layer.
  3. The red cells are washed by adding an equivalent volume of isotonic saline (9 g/L NaCl) and mixing thoroughly.
  4. Centrifuge the sample at 3000 rpm for 5 min.
  5. Label a plastic tube suitable for transport, with the patient surname and laboratory number.
  6. After centrifugation use a pasteur pipette to remove the top saline layer and 1 mm of red cells. Discard, leaving behind the washed red cells.
  7. To the labelled tube add 1 mL of washed packed red blood cells (RBC) from the middle of the washed cells.
  8. Using the same pipette tip, draw up 1 mL of distilled water and add this to the cells in the labelled tube.
  9. Stopper, mix thoroughly by rolling the haemolysate around the inside of the tube.
  10. Place the tube inside a plastic bag and freeze.

When frozen, send to laboratory.

Delphic Number Test Number3465

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