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A B C D E F G H I J K L M N O P Q

Test Identifier Information

 
Registration CodeA2
Method

Radioimmunoassay

Diagnostic Use / Indications

 

 

 

This assay is still regarded as a research procedure. The clinical applications are similar to those of renin which should normally be requested since it is easier to measure. However, if abnormalities are suspected in the generation of Angiotensin II in vivo, (excessively rare in untreated patients), assay of Angiotensin II may be preferable to PRA. Call an Endocrinologist before testing.

External Price$55.61(Exclusive of GST)
  

Specimen Collection

 
Pre-Testing Requirements

Special tube required (see Add. Info.)

Patient's posture, sodium intake, drug therapy and time of sampling all profoundly alter PRA.
Outpatients are best screened as follows:
If possible stop non-essential drugs for 2 weeks before sampling. Many hypotensive drugs alter renin levels; diuretics and ACE inhibitors increase PRA, whereas Beta-blockers reduce PRA. Alpha-blockers (Cardoxan, Prazosin and related drugs) or calcium channel blockers have less effect and are therefore preferred where the clinical condition allows.
Patients should attend (non-fasting) prior to 10.00 am for “ambulant” sampling for PRA. Plasma aldosterone also usually necessary for interpretation.
Inpatients are also screened as above and should be ambulated for at least 30 minutes before sampling. “Bed bound” patients may be sampled after a similar time in sitting position.
Other protocols involving Angiotensin II measurement include frusemide challenge, 2 hours of quiet standing or response to sodium depleting diets. Consult with an Endocrinologist for indications and test protocols.

Specimen Collection Protocols

Special tube required (see Add. Info.)

On the day of the blood collection thaw the inhibitor cocktail tubes in hot tap water and mix well. Draw a 3ml blood sample with a 5ml syringe and needle and then transfer the blood from the syringe to the AII enzyme cocktail tube. Mix the contents gently with 4 or 5 inversions. Immediately centrifuge the tube, separate the plasma and transfer the plasma to the sample tube (labelled “AII plasma only”) provided. Freeze the plasma immediately (preferably snap frozen on dry ice or liquid nitrogen). The plasma sample should then be transported frozen. Thawed samples will not be assayed.
 

Patient SpecimenBlood 3mL collected in special tube
Paediatric SpecimenBlood 2mL collected in special tube (see Add. Info.)
Sample Delivery to LabOn ice, separated immediately & frozen immediately
  

Instructions for Referral to CHLabs

 
Aliquot Instructions>0.45 mL special plasma (singleton), >1 mL special plasma. Frozen.
Aliquot Transport to CHLFrozen
  

CHLabs Laboratory

 
DepartmentEndocrinology Laboratory
Contact Details Email Email
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Contact Phone Number(03) 364 1441 | x 81441
Test AvailabilityBy arrangement only - please contact the laboratory
Turnaround TimeBy arrangement only - please contact the laboratory
Reference Interval

Ambulant, 6-24 pmol/L, 7:30am-11:30am

Interpretation

Low Angiotensin II: primary aldosteronism and other mineralocorticoid excess syndromes; "low renin" hypertension; in the aged population.
High Angiotensin II: adrenocortical insufficiency, fluid and salt wasting syndromes; Bartter’s syndrome, severe renal ischaemia, malignant hypertension, renin secreting tumours, and in young children.
Details regarding previous drug therapy (including oral contraceptives), date of last menstrual period and 24- hr urine electrolyte excretion will aid interpretation.

Uncertainty of Measurement

+/- 1.4 pmol/L for < 5.9 pmol/L

+/- 23.6% for > 5.9 pmol/L

Additional Information

Blood to be taken into 0.15mL of angiotensin inhibitor solution containing 0.125M disodium EDTA, 0.05M o-penanthroline, 0.9% sodium chloride and 2% ethanol, made up in freshly boiled distilled water with 0.2% neomycin sulphate. These tubes are available from Endolab (Phone: internal 80848, external 03-3640848).

Delphic Number Test Number6513

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