TB - PCR

Test Identifier Information
Registration Code	TBG
Method	Polymerase Chain Reaction - PCR for Mycobacterium tuberculosis is done by GeneXpert on request. Sputum - will be decontaminated before PCR testing. Pleural fluid - >5mLs undiluted or in EDTA tube. Blood or bone marrow - Collect into EDTA tube. FNA or tissue biopsy - Collect into sterile container. CSF - Minimum 1.0 mL but preferably 5.0 mLs or more (consultation with Microbiologist desirable). Seegene AnyPlex testing available for non-tuberculous mycobacteria after consultation with Microbiologist.
External Price	$214.14(Exclusive of GST)

Specimen Collection
Specimen Collection Protocols	If fresh tissue specimens are not available paraffin block specimens are acceptable. Please send 10-12 sections to the laboratory in a labelled specimen container. These are able to be tested by PCR only using AnyPlex method.
Patient Specimen	See comment.
Paediatric Specimen	As above
Sample Delivery to Lab	In labelled sterile container
Aliquot Transport to CHL	Room temperature

CHLabs Laboratory
Department	Microbiology-Bacteriology
Contact Details	Email This e-mail address is being protected from spam bots, you need JavaScript enabled to view it
Contact Phone Number	03 3640300 ext: 81356
Test Availability	Not a routine test. Batched at least weekly, urgent testing after consultation with a Microbiologist only.
Turnaround Time	8 hrs - 7 days
Reference Interval	Clinical: Microbiologist Technical: Elaine Bellerby
Additional Information	GeneXpert allows for detection of M.tuberculosis and determination of Rifampicin susceptibility from direct specimens within 24 - 48 hours of receipt in the laboratory. For M.tuberculosis on ZN positive respiratory specimens sensitivity and specificity is close to 100%. There is decreased sensitivity for ZN negative respiratory specimens and non-respiratory specimens. Seegene AnyPlex allows for detection of MTB or other (non-speciated) mycobacteria. Susceptibility testing, both genotypic and phenotypic, are completed when a M. tuberculosis culture has grown. First line susceptibilities include Rifampacin, Isoniazid, Ethambutol, Streptomycin and Pyrazinamde. For second Line susceptibilities please phone Microbiologist.
Delphic Number Test Number	8240