skip navigation
A B C D E F G H I J K L M N O P Q

Test Identifier Information

 
Registration CodeIMF
Method

Immunofixation electrophoresis is performed in four stages:

1) separation of proteins by electrophoresis on an agarose gel;

2) immunofixation (immunoprecipitation) and fixation of the electrophoresed proteins;

3) removal of unprecipitated soluble proteins by blotting and washing;, and

4) staining of the precipitated proteins for visualization.

Diagnostic Use / Indications

Immunofixation is used to determine the identity of abnormal bands visible on electrophoresis.

 

External Price$59.80(Exclusive of GST)
  

Specimen Collection

 
Specimen Collection Protocols

2 mL of blood should be collected into a plain tube (red top) and serum separated off for analysis.

Please note: PLASMA samples are unable to be accepted, the presence of fibrinogen can interfere with interpretation.

Patient SpecimenBlood 2 mL Plain (T15) (Red)
  

Instructions for Referral to CHLabs

 
Aliquot Instructions0.5 mL Serum
Aliquot Transport to CHLAmbient
  

CHLabs Laboratory

 
DepartmentBiochemistry - Protein Laboratory
Contact Details Email Email
This e-mail address is being protected from spam bots, you need JavaScript enabled to view it
Contact Phone Number(03)3640334 | x80334
Test AvailabilityMonday - Friday, 8.00 am - 3.30 pm
Turnaround Time1 - 3 days
Interpretation

No monoclonal band detected - Immunofixation did not identify any protein. There is no band visible on electrophoresis. In some instances patients with monoclonal free light chains may have no detectable monoclonal band. This is rare but has been observed.

Small band not clearly identified - A small band is evident on electrophoresis, but is not identified with immunofixation.

Small band of possible ... - A small band is identified on electrophoresis and is vaguely detected on immunofixation. Identity is uncertain.

Monoclonal band detected, identified as ... - A distinct band is identified on electrophoresis and is clearly identified on immunofixation and reported. More than one band may be identified.

Uncertainty of Measurement

Immunofixation is not a quantitative assay. If a monoclonal protein is identified, a serum protein electrophoresis assay is required for quantifying the abnormality.

Delphic Number Test Number3212

CHL Follow us on Facebook