skip navigation

Test Identifier Information

Registration CodeSPEP

As a stand alone IgM test

End-point Nephelometry: - Latex particles are coated with protein specific anti-human antibodies (e.g. anti-IgM) forming antigen:antibody complexes, when exposed to serum containing that specific protein. The latex particles improve sensitivity by increasing the turbidity of the antigen:antibody complex in solution. Quantitative nephelomtric measurement of the turbidity is proportional to the concentration of the protein in the serum. Serum protein concentrations can then be calculated by comparing the turbidity to that of a known standard. 

As part of theSerum Protein Profile (SPE)

Immunoelectrophoresis: - Electrophoretic separation of proteins in an anti-sera impregnated gel resulting in quantitative identification of specific proteins. 

Diagnostic Use / Indications

Quantitative analysis of IgM is useful for:

  • Detection and monitoring of immunodeficiency 
  • Monitoring of monoclonal gammopathies

Note:* If light chain multiple myeloma (LCMM), primary systemic amyloidosis (AL), or light chain deposition disease (LCDD) is suspected, Serum Free Light Chains(SFLC) should also be considered.

Note: Quantitative analysis may be unreliable if a cryoglobulin is suspected.

External Price$12.05(Exclusive of GST)

Specimen Collection

Patient SpecimenBlood 5-10 mL Plain(Red),Heparin(Green),EDTA(Lav).
Paediatric SpecimenBlood 0.4 mL Plain(Red),Heparin(Green) or EDTA(Lavender) ok

Instructions for Referral to CHLabs

Aliquot InstructionsMinimum 200 uL, Preferred >0.5 mL serum (preferred), or plasma. Refrigerate.
Aliquot Transport to CHLOn ice or ambient

CHLabs Laboratory

DepartmentBiochemistry - Protein Laboratory
Contact Details Email Email
This e-mail address is being protected from spam bots, you need JavaScript enabled to view it
Contact Phone Number(03)3640334 | x80334
Test AvailabilityTuesday, Thursday and Friday, 8.00 am - 4.00 pm.
Turnaround Time1 - 3 days
Reference Interval

Type and age dependant


Quantitative analysis does not distinguish between monoclonal, oligoclonal, or polyclonal elevation. Serum Protein Profile (SPE) and Immunofixation should be used to determine if any increase is due to a monoclonal band.

Note: Quantitative analysis may be unreliable if a cryoglobulin is suspected.

A monoclonal elevation of IgM is indicative of gammopathies such as:

  • Waldenstrom's macroglobulinaemia
  • Multiple myeloma
  • Monoclonal gammopathy of uncertain significance (MGUS)
  • Other related disorders.

Oligoclonal or polyclonal elevation of IgM may arise from:

  • Proliferative liver diseases (e.g. Hepatitis, cirrhosis)
  • Connective tissue diseases
  • Infections, acute and chronic.

Immunoglobulin levels may also be elevated if the blood is extracted from the umbilical cord of neonates where there has been an intrauterine or perinatal infection.

IgM deficiencies may be due to:

  • Primary or secondary immunodeficiency.
  • immunosuppression due to the presence of a plasma cell dyscrasia.
Additional Information

Refer to Protein Chemistry Lab Ext 80334.

Delphic Number Test Number3268

CHL Follow us on Facebook